UV/ozone surface modification combined with atmospheric pressure plasma irradiation for cell culture plastics to improve pluripotent stem cell culture

Culturing pluripotent stem cells effectively requires substrates coated with feeder cell layers or cell-adhesive matrices. It is difficult to employ as resources for regenerative medicine due risks of culture system contamination by animal-derived factors, the large costs associated use adhesive To enable a coating-free system, we focused on UV/ozone surface modification and atmospheric pressure plasma treatment polystyrene substrates, improve adhesion proliferation cells. In this study, develop feeder- matrix embryonic (ESCs), mouse ESCs were cultured that surface-modified using UV/ozone-plasma combined treatment. mESCs could be successfully under feeder-free conditions upon without any further chemical treatments, showed similar rates those grown layer matrix-coated substrates.